After the follicles in the ovaries reach the target size, an appropriate dose of cracking injection is administered and the egg collection phase begins after 34 - 36 hours. The egg collection phase is carried out very easily and comfortably with vaginal ultrasonography, and the person being treated will not experience any tension. The person is laid in the gynecological examination position, covered with a sterile cover, and local anesthesia is applied to the vagina, along with vaginal cleaning. Afterwards, the vaginal ultrasonography phase is started. During vaginal ultrasonography, the ovaries are reached with the help of a needle inserted into the guide on the probe. Each follicle is entered and evacuated using a special aspirator. The collected liquid is immediately sent to the laboratory and whether it contains eggs or not is checked by microscopic control. If an egg cell is found, this egg cell is removed. If an egg cannot be obtained from the follicle, the follicle cavity is washed by injecting special liquid with the same needle and they try to retrieve the egg that may be left inside. With this method, the procedures continue until all follicles are aspirated. It will take approximately 15 – 20 minutes to aspirate both eggs. After these procedures, the patient is taken to the rest room and needs to rest there for a while.
Some patients may not tolerate local anesthesia. General anesthesia may also be preferred in cases that cannot tolerate local anesthesia or in cases where the procedure is expected to be technically difficult due to the special condition of the ovaries and follicles. In some cases, although the number of follicles is high, there may be no egg cells in the follicle. One of the most important reasons for this problem, called Empty Follicle Phenomenon, is to have the patient undergo HCG and repeat the OPU procedure in the other ovary 24 hours later.
The contents of the follicle aspirated during the OPU procedure are immediately delivered to the laboratory. The egg in this liquid, which is examined through a special microscope, is placed in the culture liquid and placed in the incubator. The incubator keeps the temperature at 37 degrees and the carbon dioxide level at 5 – 6%. It helps to keep it stable. The matured egg cells become ready for fertilization after 4 to 6 hours and are suitable for use. After KOH, eggs suitable for fertilization can be obtained from approximately 80% of the follicles, which can be between 18 and 22 mm in diameter. There is no limitation regarding. Before the egg collection process, men must adjust themselves to sexual abstinence for a period not exceeding 3 - 5 days and live accordingly.
When the egg collection day comes, the man brings the sperm sample to the laboratory and delivers it, usually between 09.00 - 10.00 in the morning. It does. Considering this issue, couples who have problems in giving a sperm sample outside the home by masturbating without their spouses can also bring the sperm sample they will obtain and preserve at home to the laboratory. It must be delivered to the laboratory within minutes. For example, if it is obtained outside the laboratory environment, for the preservation method; It must be delivered to the laboratory at room temperature, without exposure to light. If this period exceeds one hour; The male sample will need to be obtained again. This situation should not be ignored or forgotten. A person should take this into consideration while making his preparations. After the sperm sample is delivered, the man must wait for the results for approximately an hour. The reason for this is that, in rare cases, a second sperm sample may be required. In this necessary case, a second sperm request is requested from the person.
In some cases, it may be possible to take a sperm sample from the testicles by aspiration or biopsy. If the inability to obtain sperm from standard semen has been previously detected, that is; In cases where sperm cannot be seen in the semen, or even if it can be seen, its quality and movement are not sufficient, urologists try to obtain a sperm sample from the testicle by needle or biopsy method. This procedure is performed by expert urologists before or on the day of egg collection.
Today's advancing technology has also provided great benefits to the field of medicine. The blessings of technology are also used during the in vitro fertilization treatment process. While testicular biopsy is performed with the latest technologies, the testicle is evaluated very well using the mapping method, and many biopsies are taken using microsurgical methods with the help of a microscope. The possibilities of obtaining sperm are also discussed in detail with the patients at the beginning of the treatment process, if desired. In addition, it is ensured that all procedures in this regard are carried out by experts in the field.
After the Egg Collection Procedure
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Usually, after an hour, the patient leaves the clinic and stays at home for a day. He needs to rest.
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You should not drive for a day, and you should not start working in daily life unless there is an urgent need.
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Some movement restrictions can also be determined according to the person's own situation.
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Although there are no restrictions on eating and drinking, of course, smoking and drinking alcohol are not appropriate in this process. It should not be consumed!
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The need for a shower or bath can be fulfilled under warm water after a day.
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Sexual intercourse is not allowed.
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The use of paracetamol and its derivative painkillers is allowed.
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The use of Crinone Gel or Progestan capsules begins on the day of egg collection.
Progesterone
With the application of progesterone, support is provided to the intrauterine layer (endometrium) and a suitable environment is created for its implantation in the uterus.
Preparations given to provide luteal support; It can be in the form of a needle (Profasi - 2000 units - 1500 units and Pluton 250 units), vaginal gel [Crinone vaginal gel] or vaginal capsule (Progestan cap). The usage and dosage of these preparations are explained to you in detail and documents are also provided. At this point, progesterone administration begins on the day of egg collection and, if pregnancy occurs, continues until the 10th week of pregnancy.
Some situations may be a red alarm. There are moments to be careful. If the following conditions are mentioned below: If any of these occur, contact your doctor immediately. These situations;
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High fever exceeding 38 C
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Excessive vaginal bleeding, higher than normal (Attention: certain It is normal to have a certain amount of bleeding)
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A very severe and constant, non-stop type of pain (Attention: it is normal to have a certain amount of pain)
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Weight gain of more than one kilo in two consecutive days,
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Inability to urinate with increasing pain, even after 8 - 10 hours after all these procedures.
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