FERTILIZATION PROCESS
Two methods are applied for this process.
1: Classical IVF Method ( In Vitro Fertilization = IVF )
It is generally preferred for couples who are young, have a high number of eggs and have good sperm characteristics. The follicle fluid, sent to the embryo laboratory in sterile tubes, is transferred to a special medium by embryologists in a special cabin, and the eggs examined under a microscope are transferred to a special medium. The eggs and sperm obtained from the sick couple are placed together in special containers prepared for this process. (Approximately 50 thousand to 100 thousand motile sperm are placed for each egg)They are kept for 16-18 hours and during this period, the sperm starts to hatch the egg on its own. fertilization is provided. After this procedure, fertilization is checked.
Even if IVF is chosen as the in vitro fertilization method, microinjection can be applied to some of the eggs to eliminate a possible fertilization failure.
2: Microinjection (Intra Cytoplasmic Sperm Injection = ICSI)
Obtaining sperm by surgical method in cases where sperm count and motility are low and deformity is high (TESA, TESE) In cases. Microinjection is preferred in cases where the number of eggs taken from the woman is insufficient, their quality is low, the egg membrane is thick, unexplained infertility cases and couples who have previously failed with the IVF method. After the sorting process, the eggs collected from the patient are cleared of the residues of the surrounding cells (cumulus cells) and the mature eggs are selected for the process. The fertilization process takes place 2-6 hours after the eggs are taken from the patient.
The sperm is injected into the egg with a needle by embryologists under a microscope using a special device called a micromanipulator. In the incubator (in a special cabinet that provides an environment similar to the mother's womb) After waiting for 16-18 hours, fertilization is checked.
Today, instead ofIVF as in vitro fertilization treatment. ICSI, that is, microinjection, is preferred. The reason for this is that the fertilization rate increases in the microinjection process. One egg Since a single sperm cell is sufficient for normal fertilization, even men with low sperm count can be allowed to have children with the microinjection method.
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