After the physicians examine the materials coming to the Pathology Laboratory, immunohistochemistry tests are performed to determine the direction of treatment.
What are the uses of immunohistochemistry?
Differential diagnosis of different tumor types and diseases such as estrogen and progesterone detection of hormone receptors Display of some gene changes (such as c-erB2…) Identification of microorganisms that cause infections (such as CMV, Hepatitis B virus…) Determination of proliferation rates (such as ki-67…) Directing the treatment (such as ERCC-1…)
Antibodies Studyed in Immunochemistry:
For Use In Cases Of Leukemia And Lymphoma And Other Hematopathology; NCL- ALK, BCL- 2, BCL - 6, CD 1a, C D 3, CD 5, CD 8, CD 15, CD 20, CD 23, CD 30, CD 31, CD 34, CD 45, CD 61, CD 68 , CD 79a, cyclin D1, kappa, lambda, myeloperoxidase, Tdt, CD 4, CD 8, CD 10, CD 15, CD 56, CD 99 and CD 23. Immature Lymphoid Markers: Tdt, CD 34,B - Cell Markers: CD 5, CD 10, CD 19, CD 20,CD 23, CD 11c, IgGkappa, IgGlambda, CD 38,T-Cell Markers: CD 3, CD 4, CD5, CD 8, CD 56, CD 57, HLA-DR .BurkittLemphoma: CD 3, CD 19, Tdt, IgGkappa, IgGlambda,NK Cell Markers: CD 16, CD 56, CD 57,Panmyeloid Markers: CD 13, CD 33,Myeloid - Granulocytic Markers: CD 15,Monocyte - Macrophage Markers : CD 14, CD 11 b, Erythroid Markers: glycophorin A, Megakaryocytic Markers: CD 41, CD 61, Others: CD 25, CD 38, HLA-DR, MDR, MPO; LCA. For Use in Unknown Tumor Phenotyping; Vimentin, chromogranin A, desmin, pancytokeratin (CK), CK 5-6, CK 7, CK 8, CK 20, e – cadherin, smooth muscle actin, muscle specificactin, myogenin, PTH, thyroglobulin, TTF-1, S-100 ,GFAP, HMB- 45, anti-Melan A, synaptophysin, CEA (mono and polyclonal), EMA, PSA, alpha 1 antitrypsin, CD 99 MIC 2, βHCG, hPL, CD 31, serotonin, ACTH, AFP, calcitonin, calretinin , GCDFP, Ca19.9, Ca 125, hepatocytes, inhibin, and Wilms tumor. Hormone Receptors, Gene Changes and As Prognostic Factors; ER, PR, c-erbB-2 /, p 53, bcl-2, cyclin D-1, Ki-67, PCNA, NCl-ALK, CD 117 (c-kit), EGfr and p 16. For identification of infectious agents ; CMV, EBV and HPV
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